The C-terminal repeat domains of nsP3 from the Old World alphaviruses bind directly to G3BP.
نویسندگان
چکیده
The Old World alphaviruses block stress granule assembly by sequestration of RasGAP SH3-domain binding protein (G3BP). Here, we show that the proline-rich sequences in the hypervariable domain of nonstructural protein 3 (nsP3) of both Semliki Forest virus and Chikungunya virus were dispensable for binding to G3BP. nsP3 variants with or without this domain colocalized with G3BP. Furthermore, we show that the C-terminal repeat motifs of nsP3 were sufficient for G3BP binding.
منابع مشابه
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عنوان ژورنال:
- Journal of virology
دوره 88 10 شماره
صفحات -
تاریخ انتشار 2014